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CONSTRUCTION OF A EUKARYOTIC EXPRESSION VECTOR FOR PEGFP-FST AND ITS BIOLOGICAL ACTIVITY IN DUCK MYOBLASTS

Abstract

Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine™ 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN).

Results
The duck pEGFP-FST vector was successfully constructed and was confirmed to possess high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a better biological activity. Significantly, the overexpression of FST in these cells significantly inhibited the mRNA expression of MSTN (a target gene that’s negatively regulated by FST).

Conclusions
The duck pEGFP-FST vector has been constructed successfully and exhibits biological activity by promoting myoblast proliferation and differentiation in vitro.

Keywords
  • pEGFP-FST vector,
  • eukaryotic expression,
  • mRNA expression
References

Authors

  • DR.DABPRY; PRO. MICHOULE SHINR
    Affiliation:- a:1:{s:5:"en_US";s:89:"Faculty in the department of biotechnology and microbiology in the university of Nigeria.";}

How to Cite

PRO. MICHOULE SHINR, D. (2021). CONSTRUCTION OF A EUKARYOTIC EXPRESSION VECTOR FOR PEGFP-FST AND ITS BIOLOGICAL ACTIVITY IN DUCK MYOBLASTS. International Journal of Multidisciplinary Research and Studies, 4(01). Retrieved from https://ijmras.com/index.php/ijmras/article/view/90

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