Abstract

Nanotechnology's game-changing applications have boosted the importance of synthesizing nanomaterials with carefully controlled dimensions. Biosynthesis of nanomaterials is a developing field that seeks to synthesize various nanoparticles.  Leaf extract from Azadirachta indica will be used to bio-generate silver nanoparticles on mesoporous silica spheres for this study. The leaf extract will be processed using ethanol. A modified version of Stober's method will be used to synthesize mesoporous silica balls. Spectroscopy, x-ray diffraction, scanning electron microscopy, and other cutting-edge tools will be employed to characterize the produced silica-supported silver nanoparticles. The disc diffusion method or the Agar well diffusion method will be used to evaluate the antibacterial efficacy of the synthesized nanoparticles. 

Keywords

• (TEOS) Tetra ethyl orthosilicate,
• (TMB) Trimethyl benzene,
• (CTAB) Hexadecyl trimethylammonium bromide,
• (MSNs) mesoporous silica nanospheres,
• (NPs) nanoparticles, (FTIR) Fourier transform infrared spectroscopy,
• (UV VISI) Ultra violet,
• (XRD) X –Ray diffraction,
• (SEM) Scanning electron microscopy

References

• Abid, N., Khan, A. M., Shujait, S., Chaudhary, K., Ikram, M., Imran, M., Haider, J., Khan, M., Khan, Q., & Maqbool, M. (2022). Synthesis of nanomaterials using various top-down and bottom-up approaches, influencing factors, advantages, and disadvantages: A review. Advances in Colloid and Interface Science, 300, 102597.
• • Ahmadi, O., Jafarizadeh-Malmiri, H., & Jodeiri, N. (2018). Eco-friendly microwave-enhanced green synthesis of silver nanoparticles using Aloe vera leaf extract and their physico-chemical and antibacterial studies. Green Processing and Synthesis, 7(3), 231-240.
• • Alahmad, A., Al-Zereini, W. A., Hijazin, T. J., Al-Madanat, O. Y., Alghoraibi, I., Al-Qaralleh, O., Al-Qaraleh, S., Feldhoff, A., Walter, J.-G., & Scheper, T. (2022). Green synthesis of silver nanoparticles using Hypericum perforatum L. aqueous extract with the evaluation of its antibacterial activity against clinical and food pathogens. Pharmaceutics, 14(5), 1104.
• • Alsammarraie, F. K., Wang, W., Zhou, P., Mustapha, A., & Lin, M. (2018). Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids and Surfaces B: Biointerfaces, 171, 398-405.
• • Asif, M., Yasmin, R., Asif, R., Ambreen, A., Mustafa, M., & Umbreen, S. (2022). Green Synthesis of Silver Nanoparticles (AgNPs), Structural Characterization, and their Antibacterial Potential. Dose-Response, 20(2), 15593258221088709.
• • Balamurugan, M., & Saravanan, S. (2017). Green synthesis of silver nanoparticles by using Eucalyptus Globulus leaf extract. Journal of The Institution of Engineers (India): Series A, 98, 461-467.
• • Banerjee, P., Satapathy, M., Mukhopahayay, A., & Das, P. (2014). Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresources and Bioprocessing, 1, 1-10.
• • CG Kiruba Daniel, S., Nehru, K., & Sivakumar, M. (2012). Rapid biosynthesis of silver nanoparticles using Eichornia crassipes and its antibacterial activity. Current Nanoscience, 8(1), 125-129.
• • Chakravarty, A., Ahmad, I., Singh, P., Sheikh, M. U. D., Aalam, G., Sagadevan, S., & Ikram, S. (2022). Green synthesis of silver nanoparticles using fruits extracts of Syzygium cumini and their bioactivity. Chemical Physics Letters, 795, 139493.
• • Chand, K., Abro, M. I., Aftab, U., Shah, A. H., Lakhan, M. N., Cao, D., Mehdi, G., & Mohamed, A. M. A. (2019). Green synthesis characterization and antimicrobial activity against Staphylococcus aureus of silver nanoparticles using extracts of neem, onion and tomato. RSC advances, 9(30), 17002-17015.
• • Deivanathan, S. K., & Prakash, J. T. J. (2022). Green synthesis of silver nanoparticles using aqueous leaf extract of Guettarda Speciosa and its antimicrobial and anti-oxidative properties. Chemical Data Collections, 38, 100831.
• • Golabiazar, R., Othman, K. I., Khalid, K. M., Maruf, D. H., Aulla, S. M., & Yusif, P. A. (2019). Green synthesis, characterization, and investigation antibacterial activity of silver nanoparticles using Pistacia atlantica leaf extract. Bionanoscience, 9(2), 323-333.
• • Gour, A., & Jain, N. K. (2019). Advances in green synthesis of nanoparticles. Artificial cells, nanomedicine, and biotechnology, 47(1), 844-851.
• • Hawar, S. N., Al-Shmgani, H. S., Al-Kubaisi, Z. A., Sulaiman, G. M., Dewir, Y. H., & Rikisahedew, J. J. (2022). Green synthesis of silver nanoparticles from Alhagi graecorum leaf extract and evaluation of their cytotoxicity and antifungal activity. Journal of Nanomaterials, 2022, 1-8.
• • Hochella Jr, M. F. (2002). Nanoscience and technology: the next revolution in the Earth sciences. Earth and Planetary Science Letters, 203(2), 593-605.
• • Jemilugba, O. T., Parani, S., Mavumengwana, V., & Oluwafemi, O. S. (2019). Green synthesis of silver nanoparticles using Combretum erythrophyllum leaves and its antibacterial activities. Colloid and Interface Science Communications, 31, 100191.
• • Khanal, L. N., Sharma, K. R., Paudyal, H., Parajuli, K., Dahal, B., Ganga, G., Pokharel, Y. R., & Kalauni, S. K. (2022). Green synthesis of silver nanoparticles from root extracts of Rubus ellipticus Sm. and comparison of antioxidant and antibacterial activity. Journal of Nanomaterials, 2022, 1-11.
• • Khane, Y., Benouis, K., Albukhaty, S., Sulaiman, G. M., Abomughaid, M. M., Al Ali, A., Aouf, D., Fenniche, F., Khane, S., & Chaibi, W. (2022). Green synthesis of silver nanoparticles using aqueous Citrus limon zest extract: Characterization and evaluation of their antioxidant and antimicrobial properties. Nanomaterials, 12(12), 2013.
• • Kingsley, J. D., Ranjan, S., Dasgupta, N., & Saha, P. (2013). Nanotechnology for tissue engineering: need, techniques and applications. journal of pharmacy research, 7(2), 200-204.
• • Kolahalam, L. A., Viswanath, I. K., Diwakar, B. S., Govindh, B., Reddy, V., & Murthy, Y. (2019). Review on nanomaterials: Synthesis and applications. Materials Today: Proceedings, 18, 2182-2190.
• • Kumar, R., Kumar, M., & Luthra, G. (2023). Fundamental approaches and applications of nanotechnology: A mini review. Materials Today: Proceedings.
• • Küp, F. Ö., Çoşkunçay, S., & Duman, F. (2020). Biosynthesis of silver nanoparticles using leaf extract of Aesculus hippocastanum (horse chestnut): Evaluation of their antibacterial, antioxidant and drug release system activities. Materials Science and Engineering: C, 107, 110207.
• • Lalitha, A., Subbaiya, R., & Ponmurugan, P. (2013). Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. Int J Curr Microbiol App Sci, 2(6), 228-235.
• • Manik, U., Nande, A., Raut, S., & Dhoble, S. (2020). Green synthesis of silver nanoparticles using plant leaf extraction of Artocarpus heterophylus and Azadirachta indica. Results in Materials, 6, 100086.
• • Mehmood, A., Ghafar, H., Yaqoob, S., Gohar, U. F., & Ahmad, B. (2017). Mesoporous silica nanoparticles: a review. J. Dev. Drugs, 6(02).
• • Misra, R., Acharya, S., & Sahoo, S. K. (2010). Cancer nanotechnology: application of nanotechnology in cancer therapy. Drug discovery today, 15(19-20), 842-850.
• • Mody, V. V., Siwale, R., Singh, A., & Mody, H. R. (2010). Introduction to metallic nanoparticles. Journal of Pharmacy and bioallied sciences, 2(4), 282.
• • Mudhafar, M., Zainol, I., Jaafar, C. N. A., Alsailawi, H., & Majhool, A. A. (2020). Microwave-assisted green synthesis of Ag nanoparticles using leaves of Melia Dubia (Neem) and its antibacterial activities. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 65(1), 121-129.
• • Nazeruddin, G., Prasad, N., Waghmare, S., Garadkar, K., & Mulla, I. (2014). Extracellular biosynthesis of silver nanoparticle using Azadirachta indica leaf extract and its anti-microbial activity. Journal of Alloys and Compounds, 583, 272-277.
• • Peralta-Videa, J. R., Huang, Y., Parsons, J. G., Zhao, L., Lopez-Moreno, L., Hernandez-Viezcas, J. A., & Gardea-Torresdey, J. L. (2016). Plant-based green synthesis of metallic nanoparticles: scientific curiosity or a realistic alternative to chemical synthesis? Nanotechnology for Environmental Engineering, 1, 1-29.
• • Rajkumar, P., Prakasam, A., Rajeshkumar, S., Gomathi, M., Anbarasan, P., & Chandrasekaran, R. (2020). Green synthesis of silver nanoparticles using Gymnema sylvestre leaf extract and evaluation of its antibacterial activity. South African Journal of Chemical Engineering, 32(1), 1-4.
• • Rangasamy, M. (2011). Nano technology: a review. Journal of applied pharmaceutical science(Issue), 08-16.
• • Rao, B., & Tang, R.-C. (2017). Green synthesis of silver nanoparticles with antibacterial activities using aqueous Eriobotrya japonica leaf extract. Advances in natural sciences: Nanoscience and nanotechnology, 8(1), 015014.
• • Roy, P., Das, B., Mohanty, A., & Mohapatra, S. (2017). Green synthesis of silver nanoparticles using Azadirachta indica leaf extract and its antimicrobial study. Applied Nanoscience, 7(8), 843-850.
• • Salayová, A., Bedlovičová, Z., Daneu, N., Baláž, M., Lukáčová Bujňáková, Z., Balážová, Ľ., & Tkáčiková, Ľ. (2021). Green synthesis of silver nanoparticles with antibacterial activity using various medicinal plant extracts: Morphology and antibacterial efficacy. Nanomaterials, 11(4), 1005.
• • Salunke, B. K., Shin, J., Sawant, S. S., Alkotaini, B., Lee, S., & Kim, B. S. (2014). Rapid biological synthesis of silver nanoparticles using Kalopanax pictus plant extract and their antimicrobial activity. Korean Journal of Chemical Engineering, 31, 2035-2040.
• • Sana, S. S., & Dogiparthi, L. K. (2018). Green synthesis of silver nanoparticles using Givotia moluccana leaf extract and evaluation of their antimicrobial activity. Materials Letters, 226, 47-51.
• • Senthilkumar, P., Rashmitha, S., Veera, P., Ignatious, C. V., SaiPriya, C., & Samrot, A. V. (2018). Antibacterial activity of neem extract and its green synthesized silver nanoparticles against Pseudomonas aeruginosa. Journal of Pure and Applied Microbiology, 12(2), 969-974.
• • Sun, Q., Cai, X., Li, J., Zheng, M., Chen, Z., & Yu, C.-P. (2014). Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloids and surfaces A: Physicochemical and Engineering aspects, 444, 226-231.
• • . S. (2016). Controllable synthesis of silver nanoparticles using Neem leaves and their Verma, A., & Mehata, M antimicrobial activity. Journal of radiation Research and applied sciences, 9(1), 109-115.
• • Widatalla, H. A., Yassin, L. F., Alrasheid, A. A., Ahmed, S. A. R., Widdatallah, M. O., Eltilib, S. H., & Mohamed, A. A. (2022). Green synthesis of silver nanoparticles using green tea leaf extract, characterization and evaluation of antimicrobial activity. Nanoscale Advances, 4(3), 911-915.
• • Yadav, S. G., Patil, S. H., Patel, P., Nair, V., Khan, S., Kakkar, S., & Gupta, A. D. (2018). Green synthesis of silver nanoparticles from plant sources and evaluation of their antimicrobial activity. Adv. Anal. Tools Mater. Charact, 5, 133-139.
• • Yousaf, H., Mehmood, A., Ahmad, K. S., & Raffi, M. (2020). Green synthesis of silver nanoparticles and their applications as an alternative antibacterial and antioxidant agent. Materials Science and Engineering: C, 112, 110901.

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