With this work, the environmentally friendly manufacturing of ZnO NPs utilizing Catharanthus roseus leaves has been assessed. Here, a rapid and environmentally friendly method for producing zinc oxide nanoparticles is described: using Catharanthus roseus leaf extract as a stabilizing and reducing agent. Different methods, including UV-visible spectroscopy, Fourier Transmission Spectroscopy (FTIR), and X-ray Diffraction (XRD) were used to analyze the green synthesized ZnO NPs. The size, morphology, and crystallinity of the synthesized ZnO nanoparticles were investigated via X-ray diffraction technique. It was found that ZnO possesses a crystallinity structure with a 24.0 nm average size. The designed molecules exhibited good antibacterial activities against gram-positive (Bacillus subtilis) gram-negative (Escherichia coli) and (Pseudomonas aeruginosa) bacterial strains. In the present work, (NE-SMS-OA-01) have been synthesized by utilizing two two-step reaction by exhibited significant antibacterial activity.
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