Abstract

Papaya leaf metabolic root pull (extract) (PLE) at a cluster of 0 (CON), 5 (LLE), 10 (MLE) and 15 (HLE) mg/250 mg dry matter (DM)with 30 mL buffered quid fluid were incubated for 24h to spot its effect on in vitro ruminal methanogenesis and ruminal bio hydrogenation (BH). Total gas production wasn't affected (P>0.05) by the addition of PLE compared to the CON at 24 h of incubation. Methane (CH4) production (mL/250mg DM) decreased (P<0.05) with increasing levels of PLE. The acetate to propionate ratio was lower (P<0.05) in MLE (2.02) and HLE (1.93) compared to the CON (2.28). Supplementation of the diet with PLE significantly (P<0.05) decreased the speed of BH of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), C18:3n-3(linolenic acid; LNA), and C18 polyunsaturated fatty acids (PUFA) compared to CON after 24 h incubation, which resulted in higher concentrations of BH intermediates like C18:1t11 (vaccenic acid; VA), c9t11 conjugated LA (CLA) (rumenic acid; RA) and t10c12 CLA. Real-time PCR analysis indicated that the entire bacteria, total protozoa, Buty-rivibrio fibri solvents, and methanogen population in HLE decreased (P<0.05) compared to CON, but the whole bacteria and B.fibri solvents population were higher (P<0.05) in CON compared to the PLE treatment

Keywords

• Bio hydrogenation, In-vitro gas production, Methanogenesis, Papaya leaf extract, Rumen fermentation

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