TY - JOUR AU - PROF. MARTHAN, PROF. HWISHART; PY - 2021/01/29 Y2 - 2024/03/28 TI - ESTABLISHMENT OF A PHEASANT (PHASIANUS COLCHICUS) SPERMATOGO-NIAL VEGETATIVE CELL LINE FOR THE ASSEMBLY OF INTERSPECIES GERMLINE CHIMERAS JF - International Journal of Multidisciplinary Research and Studies JA - ijmras VL - 4 IS - 01 SE - Articles DO - UR - https://ijmras.com/index.php/ijmras/article/view/91 SP - AB - <p><strong>Background</strong><br>Spermatogonial stem cells (SSCs) are important for&nbsp;the assembly&nbsp;of interspecies germ line chimeras. The interspecies&nbsp;cell&nbsp;transfer technique has been suggested as&nbsp;some way&nbsp;to conserve endangered birds. Our objective was to develop&nbsp;a way&nbsp;for restoring endangered birds by developing interspecies germ line chimeras between pheasant (Phasianus colchicus) and chicken (Gallus gallus) with SSCs.<br><br><strong>Results</strong><br>SSCs were isolated from the surgically removed testis of a pheasant. Growth conditions for pheasant SSCs were established by co-culturing STO (SIM mouse embryo-derived thioguanine and ouabain resistant) cells and pheasant SSCs. The colony-forming cells divided and proliferated stably to yield&nbsp;a longtime&nbsp;SSC line. Pheasant SSCs showed strong reactivity for the GDNF family receptor alpha1 (GFRα1) marker. Finally,&nbsp;the assembly&nbsp;of germ line chimeras was attempted by transferring pheasant SSCs into recipient embryos. Although final embryo survival was 5.6% (20/354), the initial survival rate was 88% (312/354).&nbsp;to live&nbsp;the percent transfer of donor SSC to gonads, the pheasant SSCs were labeled with PKH 26&nbsp;dye. We observed 30% donor cells and 9.48% c-kit/CD117-positive cells&nbsp;within the&nbsp;gonads of recipient chickens. Donor SSCs were thus stably engrafted&nbsp;within the&nbsp;recipient gonads.<br><br><strong>Conclusions</strong><br>This study showed that SSCs&nbsp;will be&nbsp;used as a tool for the conservation of endangered birds&nbsp;and also the&nbsp;production of germ line chimeras. Our findings yield insights into how we may use the pheasant spermatogonial&nbsp;vegetative cell&nbsp;line for efficient production of interspecies germ line chimeras and ultimately, to the restoration of endangered birds.</p> ER -