Abstract

Objective: The objective of the present study was to develop and characterize optimal stable nanosponges of puerarin by using the emulsion solvent evaporation technique.

 Methods: Pre-formulation studies of Peurarin were carried out. After that calibration curve was assessed for the determination of drug concentration present. The peurarin nanosponges was prepared by emulsion solvent evaporation technique (ESE-Tech) using the drug (Puerarin) 100 mg and polyvinyl alcohol (PVA) 0.3%, organic phase was prepared by dissolving ethyl cellulose (EC) (100–350 mg) and Puerarin in 20 mL dichloromethane (DCM). Fourier transform infrared spectroscopy (FTIR) estimated the compatibility of puerarin with polymer. All formulations evaluated for production yield, entrapment efficiency, in vitro drug release, scanning electron microscopy (SEM) and stability studies.

 Results: The FTIR Studies revealed that no interaction between drug and polymer. The average particle size of optimized formula (F4) was 140.8nm. High drug entrapment efficiency F4 formulation, measured at 98.73%, was possessed by the puerarin loaded nanosponges. With the help of dialysis bag diffusion method in vitro drug release studies was performed for the optimized formulations and all the dilutions measured at the wave length 244nm. At the end of 24^th hour F4 formulation shown highest drug release was found to be 97.92±1.12%. The release kinetics of the optimized formulation follows zero-order drug release. The stability study indicates no significant change in the particles size, zeta potential and entrapment efficiency of optimized formulation.

 Conclusion: The results of various evaluation parameters revealed that peurarin nanosponges would be possible alternative delivery systems to conventional formulation to improve the therapeutic value of drug.

Keywords

• Nanosponge,
• Peurarin,
• SEM,
• Ethyl cellulose,
• FTIR,
• Polymer

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