Volume 07 Issue 05

Formulation and Evaluation Puerarin Loaded Nanosponges: Formulation and Evaluation Puerarin Loaded Nanosponges


Objective: The objective of the present study was to develop and characterize optimal stable nanosponges of puerarin by using the emulsion solvent evaporation technique.

 Methods: Pre-formulation studies of Peurarin were carried out. After that calibration curve was assessed for the determination of drug concentration present. The peurarin nanosponges was prepared by emulsion solvent evaporation technique (ESE-Tech) using the drug (Puerarin) 100 mg and polyvinyl alcohol (PVA) 0.3%, organic phase was prepared by dissolving ethyl cellulose (EC) (100–350 mg) and Puerarin in 20 mL dichloromethane (DCM). Fourier transform infrared spectroscopy (FTIR) estimated the compatibility of puerarin with polymer. All formulations evaluated for production yield, entrapment efficiency, in vitro drug release, scanning electron microscopy (SEM) and stability studies.

 Results: The FTIR Studies revealed that no interaction between drug and polymer. The average particle size of optimized formula (F4) was 140.8nm. High drug entrapment efficiency F4 formulation, measured at 98.73%, was possessed by the puerarin loaded nanosponges. With the help of dialysis bag diffusion method in vitro drug release studies was performed for the optimized formulations and all the dilutions measured at the wave length 244nm. At the end of 24th hour F4 formulation shown highest drug release was found to be 97.92±1.12%. The release kinetics of the optimized formulation follows zero-order drug release. The stability study indicates no significant change in the particles size, zeta potential and entrapment efficiency of optimized formulation.

 Conclusion: The results of various evaluation parameters revealed that peurarin nanosponges would be possible alternative delivery systems to conventional formulation to improve the therapeutic value of drug.

  • Nanosponge,
  • Peurarin,
  • SEM,
  • Ethyl cellulose,
  • FTIR,
  • Polymer
  • • Vyas, S. P., & Khar, R. K. Novel Carrier Systems. Molecular Basis of Targeted Drug Delivery. Targeted and Controlled Drug Delivery, 38-40.
  • • Jilsha, G., & Viswanad, V. (2013). Nanosponges: A novel approach of drug delivery system. Int J Pharm Sci Rev Res, 19(2), 119-123.
  • • Krabicová, I., Appleton, S. L., Tannous, M., Hoti, G., Caldera, F., Rubin Pedrazzo, A., ... & Trotta, F. (2020). History of cyclodextrin nanosponges. Polymers, 12(5), 1122.
  • • Pawar, S., Shende, P., & Trotta, F. (2019). Diversity of β-cyclodextrin-based nanosponges for transformation of actives. International journal of pharmaceutics, 565, 333-350.
  • • Amin, O. M., Ammar, A., & Eladawy, S. A. (2020). Febuxostat loaded β-cyclodextrin based nanosponge tablet: An in vitro and in vivo evaluation. Journal of Pharmaceutical Investigation, 50, 399-411.
  • • Cavalli, R., Akhter, A. K., Bisazza, A., Giustetto, P., Trotta, F., & Vavia, P. (2010). Nanosponge formulations as oxygen delivery systems. International journal of pharmaceutics, 402(1-2), 254-257.
  • • Singireddy, A., Pedireddi, S. R., & Subramanian, S. (2019). Optimization of reaction parameters for synthesis of Cyclodextrin nanosponges in controlled nanoscopic size dimensions. Journal of Polymer Research, 26, 1-12.
  • • Sherje, A. P., Dravyakar, B. R., Kadam, D., & Jadhav, M. (2017). Cyclodextrin-based nanosponges: A critical review. Carbohydrate polymers, 173, 37-49.
  • • Zhou, Y. X., Zhang, H., & Peng, C. (2014). Puerarin: a review of pharmacological effects. Phytotherapy Research, 28(7), 961-975.
  • • Jain, N., & Verma, A. (2020). Preformulation studies of pilocarpine hydrochloride as niosomal gels for ocular drug delivery. Asian Journal of Pharmaceutical and Clinical Research, 149-155.
  • • Chowk, M. I. (2020). Preformulation study of terbinafine for novel drug delivery system formulation. Tablet, 250, 500mg.
  • • Kumbhar, S. C., &Salunkhe, V. R. (2013). UV Spectrophotometric Method development for Capecitabine Eudragit and Chitosan based Microspheres and its Validation. Indian Journal of Pharmaceutical and Biological Research, 1(03), 32-38.
  • • Behera, S., Ghanty, S., Ahmad, F., Santra, S., & Banerjee, S. (2012). UV-visible spectrophotometric method development and validation of assay of paracetamol tablet formulation. J Anal Bioanal Techniques, 3(6), 151-7.
  • • Chowk, M. I. (2020). Preformulation study of terbinafine for novel drug delivery system formulation. Tablet, 250, 500mg.
  • • Ahmed, M. M., Fatima, F., Anwer, M. K., Ibnouf, E. O., Kalam, M. A., Alshamsan, A., ... & Ansari, M. J. (2021). Formulation and in vitro evaluation of topical nanosponge-based gel containing butenafine for the treatment of fungal skin infection. Saudi Pharmaceutical Journal, 29(5), 467-477.
  • • Ahmed, Mohammad Muqtader, Fatima, F., Mohammed, A.B., 2020b. Olive Oil Based Organogels for Effective Topical Delivery of Fluconazole : In-vitro Antifungal Study 32, 29–36.
  • • Kumar, S., Trotta, F., & Rao, R. (2018). Encapsulation of babchi oil in cyclodextrin-based nanosponges: Physicochemical characterization, photodegradation, and in vitro cytotoxicity studies. Pharmaceutics, 10(4), 169.
  • • Penjuri, S. C. B., Ravouru, N., Damineni, S., Bns, S., &Poreddy, S. R. (2016). Formulation and evaluation of lansoprazole loaded Nanosponges. Turk. J. Pharm. Sci, 13(3), 304-310.
  • • Solunke, R. S., Borge, U. R., Murthy, K., Deshmukh, M. T., &Shete, R. V. (2019). Formulation and evaluation of gliclazide nanosponges. Int J Appl Pharm, 11(6), 181-189.
  • • Anwer, M. K., Mohammad, M., Ezzeldin, E., Fatima, F., Alalaiwe, A., & Iqbal, M. (2019). Preparation of sustained release apremilast-loaded PLGA nanoparticles: In vitro characterization and in vivo pharmacokinetic study in rats. International journal of nanomedicine, 14, 1587.
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ATALKAR VAIBHAV, A. V., & MEHTA PARULBEN D, M. P. D. (2024). Formulation and Evaluation Puerarin Loaded Nanosponges: Formulation and Evaluation Puerarin Loaded Nanosponges. International Journal of Multidisciplinary Research and Studies, 7(05), 01–15. Retrieved from

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